Biotin-Linked Polyclonal Antibody to Galectin 9 (GAL9) 
LGALS9; Ecalectin; Lectin,Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21
Overview
Properties
- Product No.LAA309Ra71
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryInfection immunity
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Rat Liver lysate
Primary Ab: 0.2μg/ml Biotin-Linked Polyclonal Antibody to Galectin 9 (GAL9)
Second Ab: 0.2μg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against GAL9. It has been selected for its ability to recognize GAL9 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Temporal Increases in Urinary Carboxymethyllysine Correlate with Albuminuria Development in DiabetesKarger: 328581
- Galectin-9 administration ameliorates CVB3 induced myocarditis by promoting the proliferation of regulatory T cells and alternatively activated Th2 cellsScienceDirect: S1521661611001057
- Rat Renal Interstitial Fibroblasts Affect the Th1/Th2 Profile In VitroPubmed: 22013937
- Serum galectin-9 levels are elevated in the patients with type 2 diabetes and chronic kidney diseasePubMed: 23339460
- Dysregulated Tim-3 expression on natural killer cells is associated with increased Galectin-9 levels in HIV-1 infectionPubMed: PMC3750478
- Upregulation of the Tim-3/Galectin-9 Pathway of T Cell Exhaustion in Chronic Hepatitis B Virus InfectionPubMed: PMC3480425
- Galectin-9 and IL-21 Mediate Cross-regulation between Th17 and Treg Cells during Acute Hepatitis CPubMed: PMC3688567
- Graves’ Disease Is Associated with a Defective Expression of the Immune Regulatory Molecule Galectin-9 in Antigen-Presenting Dendritic CellsPubMed: 25880730
- Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseasePubmed:29429028
- Galectin-9-like from Angiostrongylus cantonensis young adult worms modulates eosinophil chemotaxis in vitroDoi: 10.1016/j.jmii.2018.09.010
- The effects of Tim‑3 activation on T‑cells in gastric cancer progression
- Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra …Pubmed: 31337865
- Blood Levels of Galectin-9, an Immuno-Regulating Molecule, Reflects the Severity for the Acute and Chronic Infectious Diseases. Biomolecules 2021, 11, 43033804076
- Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …Pubmed:35687771