Biotin-Linked Polyclonal Antibody to Glutamine synthetase (GS)
GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase
Overview
Properties
- Product No.LAD761Mu71
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryEnzyme & Kinase
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against GS. It has been selected for its ability to recognize GS in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer’s diseasepubmed:27511944
- High complement levels in astrocyte‐derived exosomes of Alzheimer diseasePubmed:29406582
- Sex differences in the glutamate signaling pathway in juvenile ratsPubmed:28861894
- Deficient neurotrophic factors of CSPG4-type neural cell exosomes in Alzheimer diseasePubmed:29924942
- Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transitionPubmed: 31652385
- Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteinsPubmed: 31916313
- Decreased mitochondrial electron transport proteins and increased complement mediators in plasma neural-derived exosomes of early psychosisPubmed: 33106473
- Drug Repurposing of Asparaginase and Vitamin C Targeting Glutamine Synthetase Improves Anticancer Effect in Metastatic Castration-resistant Prostate …