Biotin-Linked Polyclonal Antibody to L1-Cell Adhesion Molecule (L1CAM)     
     
CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1
Overview
Properties
- Product No.LAA959Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
 Research use only
- Downloadn/a
- CategoryCD & Adhesion moleculeTumor immunityInfection immunityNeuro science
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against L1CAM. It has been selected for its ability to recognize L1CAM in immunohistochemical staining and western blotting.
Usage
            
                        Western blotting: 0.2-2µg/mL;1:250-2500
                        
 Immunohistochemistry: 5-20µg/mL;1:25-100
                        
 Immunocytochemistry: 5-20µg/mL;1:25-100
                        
 Optimal working dilutions must be determined by end user.
                        
        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                 Protein A/G Purification Column Protein A/G Purification Column
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                 Staining Solution for Cells and Tissue Staining Solution for Cells and Tissue
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                 Positive Control  for Antibody Positive Control  for Antibody
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                 Tissue/Sections Customized Service Tissue/Sections Customized Service
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                 Phosphorylated Antibody Customized Service Phosphorylated Antibody Customized Service
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                 Western Blot (WB) Experiment Service Western Blot (WB) Experiment Service
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                 Immunohistochemistry (IHC) Experiment Service Immunohistochemistry (IHC) Experiment Service
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                 Immunocytochemistry (ICC) Experiment Service Immunocytochemistry (ICC) Experiment Service
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                 Flow Cytometry (FCM) Experiment Service Flow Cytometry (FCM) Experiment Service
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                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
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                 Immunofluorescence (IF) Experiment Service Immunofluorescence (IF) Experiment Service
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                 Buffer Buffer
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                 DAB Chromogen Kit DAB Chromogen Kit
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                 SABC Kit SABC Kit
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                 Real Time PCR Experimental Service Real Time PCR Experimental Service
Citations
- The sL1CAM in sera of patients with endometrial and ovarian cancers.pubmed:27832351
- L1CAM overexpression downregulates phosphacan and upregulates structural plasticity-related genes rostral and caudal to the complete spinal cord transectionPubmed: 31426714
- Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial CancerPubmed: 31842290

 
         
    
     
                 
                