Biotin-Linked Polyclonal Antibody to Pleiotrophin (PTN)     
     
HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule
Overview
Properties
- Product No.LAB309Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
 Research use only
- Downloadn/a
- CategoryCytokineTumor immunityNeuro science
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against PTN. It has been selected for its ability to recognize PTN in immunohistochemical staining and western blotting.
Usage
            
                        Western blotting: 0.2-2µg/mL;1:250-2500
                        
 Immunohistochemistry: 5-20µg/mL;1:25-100
                        
 Immunocytochemistry: 5-20µg/mL;1:25-100
                        
 Optimal working dilutions must be determined by end user.
                        
        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                 Protein A/G Purification Column Protein A/G Purification Column
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                 Staining Solution for Cells and Tissue Staining Solution for Cells and Tissue
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                 Positive Control  for Antibody Positive Control  for Antibody
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                 Tissue/Sections Customized Service Tissue/Sections Customized Service
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                 Phosphorylated Antibody Customized Service Phosphorylated Antibody Customized Service
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                 Western Blot (WB) Experiment Service Western Blot (WB) Experiment Service
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                 Immunohistochemistry (IHC) Experiment Service Immunohistochemistry (IHC) Experiment Service
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                 Immunocytochemistry (ICC) Experiment Service Immunocytochemistry (ICC) Experiment Service
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                 Flow Cytometry (FCM) Experiment Service Flow Cytometry (FCM) Experiment Service
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                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
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                 Immunofluorescence (IF) Experiment Service Immunofluorescence (IF) Experiment Service
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                 Buffer Buffer
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                 DAB Chromogen Kit DAB Chromogen Kit
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                 SABC Kit SABC Kit
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                 Real Time PCR Experimental Service Real Time PCR Experimental Service
Citations
- Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophinMenin suppresses lung cancer through PTNNature: 2009273
- The Effects of Pleiotrophin in Proliferative Diabetic RetinopathyPubmed:25617851
- Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice.Pubmed:26755155
- Evaluation of Serum Biomarkers (FGF-2, HGF, MIF and PTN) in Patients With Testicular Germ Cell CancerPubmed: 31662522

 
         
    
     
                 
                