Biotin-Linked Polyclonal Antibody to Secreted Frizzled Related Protein 5 (SFRP5)
SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
- UOM
- FOB US$ 125.00 US$ 293.00 US$ 418.00 US$ 1,045.00 US$ 4,180.00
- Quantity
Overview
Properties
- Product No.LAC842Ra71
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategorySignal transduction
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against SFRP5. It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistancePubMed: 23290274
- Sfrp5 correlates with insulin resistance and oxidative stressPubMed: 23398169
- Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in childrenPubmed: 24603290
- SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in childrenPubmed: 24330025
- Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humansPubmed: 23185036
- Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery diseasePubmed: 24530778
- Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammationPubmed:Pmc4072672
- Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammationPubmed:24840810
- Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinomaPubmed:25120720
- Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controlsPubmed:25139699
- The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsisPubmed:25382802
- Circulating SFRP5 levels are elevated in drug-naPubMed: 25139699
- Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levelsPubMed: 26100762
- Sfrp5 associates with beta-cell function in humansPubmed:27019073
- Bone marrow sFRP5 level is negatively associated with bone formation markerspubmed:27986984
- Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adultspubmed:28303637
- Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 studypubmed:28851362
- Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …Pubmed:29674475
- Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican AmericansPubmed:29427376
- Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic ratsarticle-1-352-en.pdf
- The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndromePubmed: 30194556
- Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling
- A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profilePubmed: 30375290
- Role of wnt5a in Metabolic Inflammation in HumansPubmed: 30137542
- Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea PatientsPubmed: 32300333
- Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive diseasePubmed: 33188773
- Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …Pubmed: 32491086
- Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway34469882
- Association of serum uromodulin with adipokines in dependence of type 2 diabetes34920231
- Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort studyPubmed:34990470
- Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition …Pubmed:35475790
- Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …