Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) Homo sapiens (Human) Labelled antibody

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

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Overview
Properties
  • SourceAntibody labeling
  • Ig Isotype IgG, Clone Number A6
  • PurificationProtein A + Protein G affinity chromatography
  • LabelBiotin
  • Original Antibody MAA006Hu22-Monoclonal Antibody to Amphiregulin (AREG)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) Packages (Simulation)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) Packages (Simulation)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P; Sample: Human Prostate cancer Tissue; Primary Ab: 20µg/ml0Anti-Human AREG Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Skin cancer Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Colorectal cancer Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Pancreatic cancer Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) DAB staining on IHC-P;
    Sample: Human Glioma Tissue;
    Primary Ab: 20µg/ml0Anti-Human AREG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against AREG. It has been selected for its ability to recognize AREG in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological CharacteristicsKarger: 315380
  • Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.Pubmed:24800946
  • Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancerPubMed: 25707609
  • Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancerPubMed: 26195282
  • Serum level of hepatocyte growth factor is a novel marker of predicting theoutcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancerPubmed:26716644
  • Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')Pubmed: 19363516
  • CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
  • Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicinePubmed: 33212364
  • Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheetsPubmed: 33164313

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