Biotin-Linked Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
Overview
Properties
- Product No.LAB547Hu72
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseInfection immunity
- SourceAntibody labeling
- Ig Isotype IgG2b Kappa, Clone Number C13
- PurificationProtein A + Protein G affinity chromatography
- LabelBiotin
- Original Antibody RPB547Hu01-Recombinant Indoleamine-2,3-Dioxygenase (IDO)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against IDO. It has been selected for its ability to recognize IDO in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-30µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-30µg/mL
Immunohistochemistry in paraffin section: 5-30µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjectsPubmed: 21803856
- Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCTWiley: source
- Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem CellIregway: Source
- CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic MechanismsPubMed: PMC3683035
- Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinomaPubmed: 23960017
- Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocansScienceDirect: S0891584913006345
- Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiationFrontiersin:Source
- Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cellsPubmed:24813640
- The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is alteredPubmed:24947894
- Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytesPubmed:25577277
- Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose IrradiationPubMed: 26029659
- Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic propertiesHandle: 10024
- Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenasepubmed:27695127
- Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host BiocompatibilityPubmed:29883557
- Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells
- Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD MicePubmed: 30980800
- Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depressionPubmed: 31176083
- Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4
- Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡33343199
- Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4
- Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …34802039