Biotin-Linked Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)    
    
PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
- UOM
 - FOB US$ 122.00 US$ 285.00 US$ 407.00 US$ 1,018.00 US$ 4,070.00
 - Quantity
 
Overview
Properties
- Product No.LAH839Hu76
 - Organism SpeciesHomo sapiens (Human) Same name, Different species.
 - ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
 - CategoryEnzyme & Kinase
 
- SourceAntibody labeling
 - Ig Isotype IgG, Clone Number C3
 - PurificationProtein A + Protein G affinity chromatography
 - LabelBiotin
 - Original Antibody MAH839Hu26-Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)
 - Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
 - TraitsLiquid, Concentration 1mg/mL
 
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Specifity
The antibody is a mouse monoclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                    Protein A/G Purification Column
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                    Staining Solution for Cells and Tissue
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                    Positive Control  for Antibody
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                    Tissue/Sections Customized Service
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                    Phosphorylated Antibody Customized Service
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                    Western Blot (WB) Experiment Service
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                    Immunohistochemistry (IHC) Experiment Service
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                    Immunocytochemistry (ICC) Experiment Service
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                    Flow Cytometry (FCM) Experiment Service
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                    Immunoprecipitation (IP) Experiment Service
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                    Immunofluorescence (IF) Experiment Service
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                    Buffer
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                    DAB Chromogen Kit
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                    SABC Kit
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                    Real Time PCR Experimental Service
                                 
Citations
- Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
 - Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
 - Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
 - The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546
 - Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanismPubmed:29921847
 - IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancerPubmed:29465809
 - Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver …Pubmed: 32443439
 - Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancerPubmed:35689436
 
