Biotin-Linked Polyclonal Antibody to Connective Tissue Growth Factor (CTGF)
CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2
Overview
Properties
- Product No.LAA010Rb71
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryCytokine
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody PAA010Rb51-Polyclonal Antibody to Connective Tissue Growth Factor (CTGF)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against CTGF. It has been selected for its ability to recognize CTGF in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis modelPubMed: 19824055
- Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell functionPubMed: PMC2726461
- Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound HealingPlosone: 0032128
- Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathyScienceDirect: S0009912012000987
- Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?PubMed: S1053249812010121
- The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
- Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosisPubMed: 22846210
- Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosisPubMed: PMC3351780
- Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
- Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapyRo-journal: Source
- Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice ModelPubmed:Pmc4157792
- Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal modelsPubmed:24464932
- Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic LineagePubmed:24792956
- Disruption of collagen homeostasis can reverse established age-related myocardial fibrosisPubmed:25701883
- Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitisPubMed: 26190501
- Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitusPubmed:27049870
- TGF-β and Physiological Root Resorption of Deciduous Teeth.pubmed:28035998
- Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
- Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
- The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidencepubmed:27531356
- Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseasespubmed:27686729
- Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
- Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
- 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
- Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
- Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activationPubmed:29107793
- Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller CellsPubmed:30123889
- TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretionPubmed: 30426494
- Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with …Pubmed: 30413105
- Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin MPubmed: 31249305
- Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
- CTGF Attenuates Tendon-Derived Stem/Progenitor Cell AgingPubmed: 31827530
- Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced NephrotoxicityPubmed: 33212804
- Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the …Pubmed: 32943074
- MicroRNAs and ventricular remodeling in aortic stenosisPubmed: 32682570
- Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
- High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth …34455071
- Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway