Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Monoclonal antibody

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number F1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB342Mi01-Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) Packages (Simulation)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) Packages (Simulation)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P; Sample: Human Ovarian cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-Multi-species ACTa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Human Rectum Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Rat Skeletal muscle Tissue;
    Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Rat Lung Tissue;
    Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Mouse Pancreas Tissue;
    Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue;
    Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Rat Lung Tissue
    Primary Ab: 3µg/ml Mouse Anti-Multi-species ACTa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Mouse Pancreas Tissue
    Primary Ab: 3µg/ml Mouse Anti-Multi-species ACTa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue
    Primary Ab: 3µg/ml Mouse Anti-Multi-species ACTa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB342Mi21.jpg Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Bovine Heart lysate; Lane3: Canine Heart lysate Primary Ab: 0.001µg/ml Mouse Anti-Multi-species ACTa2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Rat Heart lysate; Lane3: Mouse Heart lysate
    Primary Ab: 0.005µg/ml Mouse Anti-Multi-species ACTa2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.001-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)

Giveaways

Citations

  • Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
  • Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsPubmed: 32936352
  • Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
  • Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
  • Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
  • NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network34459125

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