Monoclonal Antibody to Aquaporin 4 (AQP4) Homo sapiens (Human) Monoclonal antibody

HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number C9
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA582Hu01-Recombinant Aquaporin 4 (AQP4)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Aquaporin 4 (AQP4) Packages (Simulation)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) Packages (Simulation)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 20µg/ml Mouse Anti-Human AQP4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human AQP4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human AQP4 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) DAB staining on IHC-P;
    Sample: Human Glioma Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human AQP4 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA582Hu21.jpg Figure. Western Blot; Sample: Recombinant AQP4, Human.
  • Monoclonal Antibody to Aquaporin 4 (AQP4) Western Blot; Sample: Rat Kidney lysate;
    Primary Ab: 3µg/ml Mouse Anti-Human AQP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) Western Blot; Sample: Rat Heart lysate;
    Primary Ab: 3µg/ml Mouse Anti-Human AQP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) Western Blot; Sample: Rat Cerebrum lysate;
    Primary Ab: 3µg/ml Mouse Anti-Human AQP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Aquaporin 4 (AQP4) Western Blot; Sample: Rat Spinal cord lysate;
    Primary Ab: 3µg/ml Mouse Anti-Human AQP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against AQP4. It has been selected for its ability to recognize AQP4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.1-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patientsScienceDirect: S0304394011012341
  • Astrocytic damage is far more severe than demyelination in NMONeurology: 208
  • Aquaporin-4 expression in the cerebrospinal fluid in congenital human hydrocephalusPubMed: PMC3651869
  • Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the ratPubMed: 25616531
  • Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino ratsPubmed:26987350
  • Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertensionPubmed:26853804
  • Cerebrospinal fluid biomarkers of infantile congenital hydrocephalus.pubmed:28212403
  • Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain InjuryPubmed: 31441374
  • Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease33995926
  • Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus34597351

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