Monoclonal Antibody to Aspartate Aminotransferase (AST)
cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
Overview
Properties
- Product No.MAB214Ra22
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseTumor immunityInfection immunityHepatology
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number C6
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB214Ra01-Recombinant Aspartate Aminotransferase (AST)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Specifity
The antibody is a mouse monoclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanismsPubMed: PMC3612362
- Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse modelPubMed: PMC3382536
- Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in ratsAcademicjournals: Source
- Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanismsPubmed: 23325074
- Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in ratsScienceDirect: S0014299914000235
- The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in MicePubmed:25480563
- Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol PoisoningPubMed: 26697061
- Advanced Studies in Clinical and Experimental Research in GastroenterologyPubmed:26955389
- Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.pubmed:27678302
- Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
- Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase DeficiencyPubmed:29379059
- A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosisPubmed:29321963
- Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsPubmed: 28095910
- Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiencyPubmed: 31186526
- Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathyPubmed: 31437533
- Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
- Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
- Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
- Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus33925018
- Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes34848154
- NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury34937917
- Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
- Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and …Pubmed:35548710
- Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activationPubmed:35605433