Monoclonal Antibody to Beta-2-Microglobulin (b2M) Homo sapiens (Human) Monoclonal antibody

BMG; B2MG

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number D9
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA260Hu01-Recombinant Beta-2-Microglobulin (b2M)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) Packages (Simulation)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) Packages (Simulation)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Samples: Human Lung Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Samples: Human Heart Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Sample: Human Heart Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Sample: Human Liver Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Beta-2-Microglobulin (b2M) DAB staining on IHC-P;
    Sample: Human Lung Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human b2M Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against b2M. It has been selected for its ability to recognize b2M in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
  • Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recoveryBiomedcentral: Source
  • Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner.pubmed:28959042
  • Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
  • Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approachPubmed:29315472
  • Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
  • Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
  • iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma33540066
  • Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 146434573096
  • Biomarkers of cadmium exposure and renal function in estuarine adult villagers34773507

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