Monoclonal Antibody to Calnexin (CNX)

CANX; CNX; IP90; P90; Major Histocompatibility Complex Class I Antigen-Binding Protein p88

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 130.00 US$ 302.00 US$ 432.00 US$ 1,080.00 US$ 4,320.00
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Overview

Properties

Product No.MAA280Hu23
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Developmental science

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG2b Kappa, Clone Number C2
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPA280Hu01-Recombinant Calnexin (CNX)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10µg/ml Mouse Anti-Human CNX Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Mouse Anti-Human CNX Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 10µg/ml Mouse Anti-Human CNX Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10µg/ml Mouse Anti-Human CNX Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 40µg/ml Mouse Anti-Human CNX Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 40µg/ml Mouse Anti-Human CNX Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

WB Image
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: A549 cell lysate; Lane3: Porcine Lung lysate; Lane4: Porcine Cerebrum lysate; Lane5: Rat Lung lysate; Lane6: Rat Cerebrum lysate; Lane7: Canine Cerebrum lysate; Lane8: Bovine Lung lysate; Lane9: Bovine Cerebrum lysate
Primary Ab: 2μg/ml Mouse Anti-Human CNX Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CNX. It has been selected for its ability to recognize CNX in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)