Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) Rattus norvegicus (Rat) Monoclonal antibody

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPJ365Ra01-Recombinant Catechol-O-Methyltransferase (COMT)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) Packages (Simulation)
  • Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) Packages (Simulation)
  • Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) DAB staining on IHC-P; Sample: Rat Liver Tissue; Primary Ab: 30µg/ml Mouse Anti-Rat COMT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) DAB staining on IHC-P;
    Sample: Rat Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Rat COMT Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAJ365Ra21.jpg Western Blot; Sample: Rat Liver lysate Primary Ab: 0.2µg/ml Mouse Anti-Rat COMT Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against COMT. It has been selected for its ability to recognize COMT in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary.PubMed: 22750137

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