Monoclonal Antibody to Cluster Of Differentiation 83 (CD83)

BL11; HB15; Activated B Lymphocytes,Immunoglobulin; B-cell activation protein; Cell surface protein HB15

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 109.00 US$ 253.00 US$ 362.00 US$ 905.00 US$ 3,620.00
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Overview

Properties

Product No.MAB845Mu21
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule Infection immunity Immune molecule Dermatology

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG2b Kappa, Clone Number C10
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB845Mu01-Recombinant Cluster Of Differentiation 83 (CD83)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.82mg/ml

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Packages (Simulation)
Packages (Simulation)

Western Blot; Sample: Rat Spleen lysate Primary Ab: 3μg/ml Mouse Anti-Mouse CD83 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CD83. It has been selected for its ability to recognize CD83 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-3µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

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Citations

Murine CD83-positive T cells mediate suppressor functions in vitro and in vivoPubmed:25151500
The Polyunsaturated Fatty Acids Arachidonic Acid and Docosahexaenoic Acid Induce Mouse Dendritic Cells Maturation but Reduce T-Cell Responses In VitroPubMed: 26619195
NK cells are negatively regulated by sCD83 in experimental autoimmune uveitispubmed:29038541
A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice.pubmed:28491062
Soluble CD83 Alleviates Experimental Autoimmune Uveitis by Inhibiting Filamentous Actin-Dependent Calcium Release in Dendritic CellsPubmed:30050530
Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasionPubmed: 32810792