Monoclonal Antibody to Cofilin 1 (CFL1)

CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 109.00 US$ 255.00 US$ 364.00 US$ 910.00 US$ 3,640.00
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Overview

Properties

Product No.MAB559Hu24
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Metabolic pathway Neuro science Bone metabolism

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG2b Kappa, Clone Number C6
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB559Hu01-Recombinant Cofilin 1 (CFL1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.53mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Cardiac Muscle Tissue; Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Cerebrum lysate; Lane3: Porcine Skin lysate; Lane4: HepG2 cell lysate Primary Ab: 2µg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: HepG2 cell lysate; Lane3: MCF7 cell lysate
Primary Ab: 0.3µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CFL1. It has been selected for its ability to recognize CFL1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.3-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

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Citations

Detection of sputum cofilin-1 as indicator of malignancyPubmed:29846436
Hydroxytyrosol as a Promising Ally in the Treatment of FibromyalgiaPubmed: 32784915
The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer