Monoclonal Antibody to Collagen Type III Alpha 1 (COL3a1)     
     
COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain
Overview
Properties
- Product No.MAD195Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
 If the antibody is used in flow cytometry, please check FCM antibodies.
 Research use only
- Downloadn/a
- CategoryMetabolic pathway
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG, Clone Number n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against COL3a1. It has been selected for its ability to recognize COL3a1 in immunohistochemical staining and western blotting.
Usage
            
                        Western blotting: 0.2-2µg/mL;1:500-5000
                        
 Immunohistochemistry: 5-20µg/mL;1:50-200
                        
 Immunocytochemistry: 5-20µg/mL;1:50-200
                        
 Optimal working dilutions must be determined by end user.
                        
        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                 Antibody Labeling Customized Service Antibody Labeling Customized Service
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                 Protein A/G Purification Column Protein A/G Purification Column
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                 Staining Solution for Cells and Tissue Staining Solution for Cells and Tissue
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                 Positive Control  for Antibody Positive Control  for Antibody
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                 Tissue/Sections Customized Service Tissue/Sections Customized Service
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                 Phosphorylated Antibody Customized Service Phosphorylated Antibody Customized Service
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                 Western Blot (WB) Experiment Service Western Blot (WB) Experiment Service
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                 Immunohistochemistry (IHC) Experiment Service Immunohistochemistry (IHC) Experiment Service
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                 Immunocytochemistry (ICC) Experiment Service Immunocytochemistry (ICC) Experiment Service
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                 Flow Cytometry (FCM) Experiment Service Flow Cytometry (FCM) Experiment Service
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                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
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                 Immunofluorescence (IF) Experiment Service Immunofluorescence (IF) Experiment Service
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                 Buffer Buffer
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                 DAB Chromogen Kit DAB Chromogen Kit
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                 SABC Kit SABC Kit
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                 Long-arm Biotin Labeling Kit Long-arm Biotin Labeling Kit
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                 Real Time PCR Experimental Service Real Time PCR Experimental Service
Citations
- Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-cultureUnivmed:Source
- Epithelial but not stromal expression of collagen alpha-1 (III) is a diagnostic and prognostic indicator of colorectal carcinomaPubmed:26741506
- Association between Plasma HMGB-1 and Silicosis: A Case-Control StudyPubmed: 30558126

 
         
    
     
                 
                