Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)

COL4-A5; ASLN; ATS; CA54; Collagen Alpha-5(IV)chain; Alport Syndrome

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 107.00 US$ 249.00 US$ 355.00 US$ 888.00 US$ 3,550.00
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Overview

Properties

Product No.MAC141Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryMetabolic pathway Developmental science Nutrition metabolism

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG1 Kappa, Clone Number E3
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPC141Hu01-Recombinant Collagen Type IV Alpha 5 (COL4a5)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human COL4a5 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against COL4a5. It has been selected for its ability to recognize COL4a5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM diseasePubmed: 30817909
Basement Membrane Remodelling Controls Endothelial Function in IPAHPubmed: 32160015