Monoclonal Antibody to Complement Factor H (CFH) Rattus norvegicus (Rat) Monoclonal antibody

CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C3
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA635Ra01-Recombinant Complement Factor H (CFH)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Complement Factor H (CFH) Packages (Simulation)
  • Monoclonal Antibody to Complement Factor H (CFH) Packages (Simulation)
  • Monoclonal Antibody to Complement Factor H (CFH) DAB staining on IHC-P;
    Sample: Human Brain Tissue
    Primary Ab: 20µg/ml Mouse Anti-Rat CFH Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA635Ra21.jpg Figure. Western Blot; Sample: Recombinant CFH, Rat.
  • Monoclonal Antibody to Complement Factor H (CFH) Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Plasma
    Primary Ab: 0.2µg/ml Mouse Anti-Rat CFH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CFH. It has been selected for its ability to recognize CFH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • Circulating matrix metalloproteinases and procollagen propeptides in inguinal herniaPubmed:29484522
  • Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor HPubmed: 31187271
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H33788378
  • A Family Affair: Addressing the Challenges of Factor H and the Related Proteins33868311

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