Monoclonal Antibody to Cystatin B (CSTB) Rattus norvegicus (Rat) Monoclonal antibody

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number 1:2430000 (C13)
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPJ322Ra01-Recombinant Cystatin B (CSTB)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cystatin B (CSTB) Packages (Simulation)
  • Monoclonal Antibody to Cystatin B (CSTB) Packages (Simulation)
  • Monoclonal Antibody to Cystatin B (CSTB) DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 40µg/ml Mouse Anti-Rat CSTB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • MAJ322Ra21.jpg Western Blot; Sample: Rat Liver lysate Primary Ab: 0.2µg/ml Mouse Anti-Rat CSTB Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cystatin B (CSTB) Western Blot; Samples: Lane1: Rat Placenta lysate; Lane2: Rat Spleen lysate;
    Primary Ab: 0.2µg/ml Mouse Anti-Rat CSTB Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CSTB. It has been selected for its ability to recognize CSTB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disordersPubMed: 23291538
  • Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancerPubMed: PMC3754495
  • Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal ApoptosisPubMed: PMC3365072
  • Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophagesPubmed:25209871
  • A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive systemScience: Article
  • Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.pubmed:28766361

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