Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1) Rattus norvegicus (Rat) Monoclonal antibody

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1) Packages (Simulation)
  • Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CYP7A1. It has been selected for its ability to recognize CYP7A1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile AcidsPubmed: 30605678
  • Hyperglycemia exacerbates dyslipidemia-induced changes in uptake, synthesis, and transporters of bile acids in rats: Assessment of restorative potentials of ALA and?…Doi: 10.1016/j.jff.2019.01.038
  • Withaferin-A down-regulate enterohepatic circulation of bile acids: An insight from a hyperlipidemic rat model

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