Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) Homo sapiens (Human) Monoclonal antibody

SK; Dickkopf Homolog 1(Xenopus Laevis)

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA741Hu01-Recombinant Dickkopf Related Protein 1 (DKK1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) Packages (Simulation)
  • Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) Packages (Simulation)
  • MAA741Hu21.jpg Western Blot; Sample: Mouse Sp2/0 cell lysate; Primary Ab: 3µg/ml Mouse Anti-Human DKK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against DKK1. It has been selected for its ability to recognize DKK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian studyPubmed: 32198440
  • Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathwayPubmed: 32041953
  • Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …Pubmed: 33065276
  • Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats34373766

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