Monoclonal Antibody to Enolase 1 (ENO1) Homo sapiens (Human) Monoclonal antibody

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1Kappa, Clone Number D5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB449Hu01-Recombinant Enolase 1 (ENO1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Enolase 1 (ENO1) Packages (Simulation)
  • Monoclonal Antibody to Enolase 1 (ENO1) Packages (Simulation)
  • Monoclonal Antibody to Enolase 1 (ENO1) Western Blot; Samples: Lane1: Hela cell lysate; Lane2: PC3 cell lysate;
    Primary Ab: 0.2µg/ml Mouse Anti-Human ENO1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensorPubMed: 20557064
  • Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodulesPubmed:27172385
  • Putative salivary biomarkers useful to differentiate patients with fibromyalgiaPubmed:29654921
  • The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of EndometriosisPubmed: 33062681
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212
  • Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinomaPubmed:35610567

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