Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2) Homo sapiens (Human) Monoclonal antibody

MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2) Packages (Simulation)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2) Packages (Simulation)
  • MAA930Hu25.jpg Western Blot; Sample: Rat Kidney lysate Primary Ab: 0.2µg/ml Mouse Anti-Human ERK2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2) Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: 3T3-L1 cell lysate; Lane3: Hela cell lysate;
    Primary Ab: 0.2µg/ml Mouse Anti-Human ERK2 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ERK2. It has been selected for its ability to recognize ERK2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s DiseasePubmed:26119304
  • Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
  • Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317

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