Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) Homo sapiens (Human) Monoclonal antibody

FTH1; FTHL6; PIG15; PLIF; Ferritin Heavy Chain; Apoferritin; Placenta Immunoregulatory Factor; Proliferation-Inducing Protein 15; Cell proliferation-inducing gene 15 protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number D4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPD021Hu01-Recombinant Ferritin, Heavy Polypeptide (FTH)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) Packages (Simulation)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) Packages (Simulation)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P;
    Samples: Human Spleen Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P;
    Sample: Human Liver Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) DAB staining on IHC-P;
    Sample: Human Spleen Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human FTH Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAD021Hu21.jpg Figure. Western Blot; Sample: Recombinant FTH, Human.
  • Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Kidney lysate; Lane4: U87MG cell lysate
    Primary Ab: 2µg/ml Mouse Anti-Human FTH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against FTH. It has been selected for its ability to recognize FTH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Presence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical OnePubMed: PMC3765839
  • Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cellsPubmed:24142518
  • The Oral Iron Chelator, Deferasirox, Reverses the Age-Dependent Alterations in Iron and Amyloid-β Homeostasis in Rat Brain: Implications in the Therapy of Alzheimer’s DiseasePubMed: 26484920
  • MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoterPubmed:26960542
  • Impact of TET2 deficiency on iron metabolism in erythroblasts.pubmed:28167288

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