Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) Mus musculus (Mouse) Monoclonal antibody

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C14
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPC343Mu01-Recombinant Heat Shock 70kDa Protein 5 (HSPA5)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 20µg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) FITC staining on IF;
    Sample: Human Hela cell;
    Primary Ab: 30ug/ml Mouse Anti-Mouse HSPA5 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against HSPA5. It has been selected for its ability to recognize HSPA5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes.PubMed: 20522592
  • Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptorsPubmed: 31048034
  • Fenfluramine acts as a positive modulator of sigma-1 receptorsPubmed: 32169824
  • Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer33381181
  • Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior
  • Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients34414940

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