Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Homo sapiens (Human) Monoclonal antibody

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

Add to Cart Distributors
Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA693Hu01-Recombinant Heat Shock Protein 27 (Hsp27)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 30µg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Sample: Human Glioma Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA693Hu23.jpg Western Blot; Sample: Human Lung lysate; Primary Ab: 2µg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Porcine Skeletal muscle lysate;
    Primary Ab: 2µg/ml Mouse Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Porcine Heart lysate;
    Primary Ab: 2µg/ml Mouse Anti-Human HSPB1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
    Primary Ab: 0.2µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock Protein 27 (Hsp27) Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog MAA693Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cellsPubMed: 26467738
  • HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosisPubmed:29650953
  • Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseasePubmed:29429028
  • The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacityPubmed: 31102684
  • Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …Pubmed:35687771
  • Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation

Recommend products