Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) Homo sapiens (Human) Monoclonal antibody

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1Kappa, Clone Number C5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA822Hu01-Recombinant Heat Shock Protein 60 (Hsp60)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Mouse Anti-Human Hsp60 Antibody
    Second Ab: 2µg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.

Usage

Immunofluorescence: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Hsp60 response in experimental and human temporal lobe epilepsyPubMed: 25801186
  • Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitroPubmed: 30878663
  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970

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