Monoclonal Antibody to Heparanase (HPSE) 
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
Overview
Properties
- Product No.MAA711Ra21
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & Kinase
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C303
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA711Ra01-Recombinant Heparanase (HPSE)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Sample: Human Hela cell; Primary Ab: 30ug/ml Mouse Anti-Rat HPSE Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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Western Blot; Sample: Jurkat cell lysate Primary Ab: 0.5µg/ml Mouse Anti-Rat HPSE Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against HPSE. It has been selected for its ability to recognize HPSE in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Homo sapiens (Human)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma ModelPlosone: Source
- Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproductionNowinylekarskie:Source
- Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanasePubMed: 26191183
- PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resectionPubMed: 26415733
- Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproductionarticle:84
- Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage pubmed:28994624