Monoclonal Antibody to Immunoglobulin G4 (IgG4) Homo sapiens (Human) Monoclonal antibody

IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA234Hu01-Recombinant Immunoglobulin G4 (IgG4)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • Monoclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • MAA234Hu21.jpg Western Blot; Sample: Human Serum Primary Ab: 2μg/ml Mouse Anti-Human IgG4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitisPubmed:25194335
  • Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 CellsPubmed:25561924
  • Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s diseasePubMed: 26377996
  • The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394
  • Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patientsarticle:30785

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