Monoclonal Antibody to Interferon Gamma (IFNg)    
    
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
Overview
Properties
- Product No.MAA049Ra21
 - Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
 - ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
 - CategoryCytokineInfection immunity
 
- SourceMonoclonal antibody preparation, Host Mouse
 - Ig Isotype IgG2a Kappa, Clone Number B7
 - PurificationProtein A + Protein G affinity chromatography
 - LabelNone
 - Immunogen RPA049Ra01-Recombinant Interferon Gamma (IFNg)
 - Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
 - TraitsLiquid, Concentration 1mg/ml
 
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Specifity
The antibody is a mouse monoclonal antibody raised against IFNg. It has been selected for its ability to recognize IFNg in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                    Antibody Labeling Customized Service
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                    Protein A/G Purification Column
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                    Staining Solution for Cells and Tissue
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                    Positive Control  for Antibody
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                    Tissue/Sections Customized Service
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                    Phosphorylated Antibody Customized Service
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                    Western Blot (WB) Experiment Service
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                    Immunohistochemistry (IHC) Experiment Service
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                    Immunocytochemistry (ICC) Experiment Service
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                    Flow Cytometry (FCM) Experiment Service
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                    Immunoprecipitation (IP) Experiment Service
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                    Immunofluorescence (IF) Experiment Service
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                    Buffer
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                    DAB Chromogen Kit
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                    SABC Kit
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                    Long-arm Biotin Labeling Kit
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                    Real Time PCR Experimental Service
                                 
Citations
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 - Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
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 - Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in?Escherichia coli?induce potent protective immune responses in guinea pigs, swine and cattlePubMed: PMC3720265
 - Immune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against CoccidiosisPjbs: Source
 - Protective effects of neferine on amiodarone-induced pulmonary fibrosis in micePubmed: 23792144
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 - Exogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in micePubmed:25724769
 - Protective effect and mechanism of probucol in the treatment of spinal cord injuryPubMed: 26214485
 - The toxic effect of nickel nanoparticles on oxidative stress and inflammatory markers.Articles:
 - Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbuminPubMed: 25760938
 - Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsArticle_14936_6.Html:
 - Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE12 and is crucial for liver transplant tolerancePubMed: 25982596
 - Trypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in micePubMed: 25912691
 - Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
 - The immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
 - Nanogel‐Incorporated Physical and Chemical Hybrid Gels for Highly Effective Chemo–Protein Combination TherapyDoi: 10.1002
 - Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSFPubMed: 26300317
 - Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4PubMed: 26573719
 - Possible Association of IFN-γ GenePubMed: 26154533
 - Rituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphomaPubMed: 26315113
 - Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunityPubMed: 26607265
 - INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATSjournals:3790
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 - Reno-protective effect and mechanism study of Huang Lian Jie Du Decoction on lupus nephritis MRL/lpr micepubmed:27825337
 - Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.pubmed:28366526
 - Evaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus.pubmed:27908832
 - Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.pubmed:28049588
 - Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.pubmed:27867030
 - Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation09540105.2017.1306494
 - Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathologypubmed:27830033
 - Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthmapubmed:27289030
 - EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILER363-379.pdf
 - Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
 - Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) andprobiotics on immune responses and caecal counts in young laying hens.pubmed:27678135
 - Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice.pubmed:28134069
 - Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus.pubmed:28063474
 - Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.pubmed:28220870
 - Assessment of the efficacy of two novel DNA vaccine formulations against highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus.pubmed:28157199
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 - The effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+ and CD8+ T …S0719-81322017000300185
 - Evaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCRDOI: 10.18869/acadpub.rmm.5.2.3
 - Stress Induced Polarization of Immune-Neuroendocrine Phenotypes in Gallus galluspubmed:28808318
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 - Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
 - An attenuated Shigella mutant lacking the RNA-binding protein Hfq provides cross-protection against Shigella strains of broad serotypepubmed:28727722
 - Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells10.18632/oncotarget.15445
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 - EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-ݜ傠Signaling Pathway in Rats10.1155/2017/3057268
 - Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ.pubmed:28649126
 - EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats10.1155/2017/3057268
 - Associations among interleukin-17, interferon-gamma, and acute coronary syndromeISSN 0970-938X
 - Double-Edged Effect of Hydroxychloroquine on Human Umbilical Cord-Derived Mesenchymal Stem Cells Treating Lupus Nephritis in MRL/lpr MicePubmed:29668284
 - Sonodynamic therapy‐assisted immunotherapy: A novel modality for cancer treatmentPubmed:29575297
 - Identification and preliminary study of immunogens involved in autoimmune prostatitis in human malesPubmed:29947032
 - Immunogenicity and protective efficacy of recombinant fiber-2 protein in protecting SPF chickens against fowl adenovirus 4Pubmed:29395523
 - Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cellsPubmed:29989653
 - Epstein Barr Virus Infection Affects Function of Cytotoxic T Lymphocytes in Patients with Severe Aplastic AnemiaPubmed:29862282
 - Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
 - Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
 - Immunomodulatory activity of Lactobacillus plantarum KLDS1. 0318 in cyclophosphamide-treated micePubmed:30026678
 - Involvement of T-Helper 9 Activation in a Mouse Model of Allergic RhinitisPubmed:29982264
 - Proinflammatory Cytokine Production by CD-1 Mice in Response to Equipotential Doses of PG and …10.1134:S0006297918070088
 - Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroPubmed:30127867
 - Increased Macroautophagy in Interferon-Gamma-Producing T Cells from Patients with Newly Diagnosed Systemic Lupus ErythematosusPubmed:29941705
 - Construction of Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific …10.1016:S2095-3119(17)61696-4
 - Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
 - Effect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentrationPubmed:29423707
 - Immune response characteristics of Capri pox virus vaccines following emergency vaccination of cattle against lumpy skin disease virus65381
 - HUVEC 疫苗联合化疗抗EMT-6 乳腺癌转移实验研究Immune99:Source
 - خصوصیات پاسخهای ایمنی القاء شده ناشی از واکسن زنده تخفیف حدت یافته آبله بزی سویه گرگان بر علیه بیماری لمپی اسکین در گاو
 - Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387
 - PD-1 blockade inhibits lymphocyte apoptosis and restores proliferation and anti-viral immune functions of lymphocyte after CP and NCP BVDV infection in vitroDoi: 10.1016/j.vetmic.2018.10.014
 - NK Cells Suppress CD8+ T Cell Immunity via NKG2D in Severe Aplastic AnemiaDoi: 10.1016/j.cellimm.2018.10.004
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