Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Homo sapiens (Human) Monoclonal antibody

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number C12
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPH839Hu01-Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Figure. DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Figure. DAB staining on IHC-P;
    Samples: Human Glioma Tissue.
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Figure. DAB staining on IHC-P;
    Samples: Human Kidney Tissue.
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Figure. DAB staining on IHC-P;
    Samples: Human Stomach Tissue.
  • MAH839Hu24.jpg Figure. Western Blot; Sample: Recombinant IDH1, Human.
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Figure. Western Blot; Sample: Lane1: Human Liver Tissue; Lane2: Porcine Liver Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Citations

  • Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
  • Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
  • Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
  • The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546

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