Monoclonal Antibody to Cytokeratin 4 (CK4) 
CYK4; KRT4; K4; Keratin 4; Keratin, type II cytoskeletal 4; Type-II keratin Kb4
Overview
Properties
- Product No.MAA489Ra21
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryTumor immunity
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number 1:270000 (1#)
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA489Ra01-Recombinant Cytokeratin 4 (CK4)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Packages (Simulation)
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Packages (Simulation)
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Figure.Western Blot;Sample: Recombinant KRT4, Rat.
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against CK4. It has been selected for its ability to recognize CK4 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranesPubmed:29749501
- Development of a novel murine heart failure model overexpressing human renin and angiotensinogenPubmed: 32056390
- Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal …Pubmed:35451104