Monoclonal Antibody to Lymphocyte Activation Gene 3 (LAG3)

CD223; Protein FDC

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 106.00 US$ 246.00 US$ 352.00 US$ 880.00 US$ 3,520.00
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Overview

Properties

Product No.MAB663Hu21
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryTumor immunity Infection immunity Immune molecule

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG1 Kappa, Clone Number C1
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB663Hu01-Recombinant Lymphocyte Activation Gene 3 (LAG3)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Mouse Anti-Human LAG3 Antibody Second Ab: 3μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)

Western Blot; Sample: Lane1: Jurkat cell lysate; Lane2: K562 cell lysate; Lane3: HepG2 cell lysate Primary Ab: 4μg/ml Mouse Anti-Human LAG3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against LAG3. It has been selected for its ability to recognize LAG3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-4µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

A signal-decreased electrochemical immunosensor for the sensitive detection of LAG-3 protein based on a hollow nanobox-MOFs/AuPt alloyPubmed:29772383
Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387