Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)     
     
ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein
Overview
Properties
- Product No.MAC300Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsIHC
 If the antibody is used in flow cytometry, please check FCM antibodies.
 Research use only
- DownloadInstruction Manual
- CategoryTumor immunityNeuro science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number C2
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPC300Hu01-Recombinant Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against MANF. It has been selected for its ability to recognize MANF in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                 Antibody Labeling Customized Service Antibody Labeling Customized Service
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                 Protein A/G Purification Column Protein A/G Purification Column
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                 Staining Solution for Cells and Tissue Staining Solution for Cells and Tissue
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                 Positive Control  for Antibody Positive Control  for Antibody
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                 Tissue/Sections Customized Service Tissue/Sections Customized Service
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                 Phosphorylated Antibody Customized Service Phosphorylated Antibody Customized Service
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                 Western Blot (WB) Experiment Service Western Blot (WB) Experiment Service
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                 Immunohistochemistry (IHC) Experiment Service Immunohistochemistry (IHC) Experiment Service
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                 Immunocytochemistry (ICC) Experiment Service Immunocytochemistry (ICC) Experiment Service
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                 Flow Cytometry (FCM) Experiment Service Flow Cytometry (FCM) Experiment Service
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                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
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                 Immunofluorescence (IF) Experiment Service Immunofluorescence (IF) Experiment Service
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                 Buffer Buffer
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                 DAB Chromogen Kit DAB Chromogen Kit
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                 SABC Kit SABC Kit
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                 Long-arm Biotin Labeling Kit Long-arm Biotin Labeling Kit
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                 Real Time PCR Experimental Service Real Time PCR Experimental Service
Citations
- Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
- Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.pubmed:28216543
- Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
- Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance10.1507:endocrj.EJ16-0472
- MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage
- Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway33856409
- The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …34234505

 
         
    
     
                 
                 
                                