Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Homo sapiens (Human) Monoclonal antibody

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) AF488 staining on IF;
    Sample: HepG2 cell
    Primary Ab: 30µg/ml Mouse Anti-Human MANF Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against MANF. It has been selected for its ability to recognize MANF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.pubmed:28216543
  • Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance10.1507:endocrj.EJ16-0472
  • MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage
  • Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway33856409
  • The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …34234505

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