Monoclonal Antibody to Nephrin (NPHN) Rattus norvegicus (Rat) Monoclonal antibody

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C2
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA937Ra01-Recombinant Nephrin (NPHN)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Nephrin (NPHN) Packages (Simulation)
  • Monoclonal Antibody to Nephrin (NPHN) Packages (Simulation)
  • Monoclonal Antibody to Nephrin (NPHN) DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 20µg/ml Mouse Anti-Rat NPHN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • MAA937Ra21.jpg Figure. Western Blot; Sample: Recombinant NPHN, Rat.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia.pubmed:28120572
  • Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.pubmed:29246788
  • Renal protection by atorvastatin in a murine model of sickle cell nephropathyPubmed:29527679
  • Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney DiseasePubmed: 32961903
  • Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study34707724
  • Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugsPubmed:35620307

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