Monoclonal Antibody to Neuraminidase (NEU) Homo sapiens (Human) Monoclonal antibody

NEU1; SIAL1; Sialidase 1; Lysosomal Sialidase; Acetylneuraminyl hydrolase; G9 sialidase; N-acetyl-alpha-neuraminidase 1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number 2#
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB611Hu02-Recombinant Neuraminidase (NEU)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Neuraminidase (NEU) Packages (Simulation)
  • Monoclonal Antibody to Neuraminidase (NEU) Packages (Simulation)
  • Monoclonal Antibody to Neuraminidase (NEU) DAB staining on IHC-P;
    Sample: Porcine Cerebrum Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human NEU Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Neuraminidase (NEU) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human NEU Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB611Hu21.jpg Figure. Western Blot; Sample: Recombinant NEU, Human.
  • Monoclonal Antibody to Neuraminidase (NEU) Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: LO2 cell lysate;
    Primary Ab: 1µg/ml Mouse Anti-Human NEU Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against NEU. It has been selected for its ability to recognize NEU in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • METHODS OF PREVENTING OR TREATING ATHEROSCLEROSIS WITH INHIBITORS OF SPECIFIC ISOENZYMES OF HUMAN NEURAMINIDASE

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