Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
Overview
Properties
- Product No.MAE038Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryNeuro science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number C4
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPE038Hu02-Recombinant Neurofilament, Light Polypeptide (NEFL)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Specifity
The antibody is a mouse monoclonal antibody raised against NEFL. It has been selected for its ability to recognize NEFL in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.001-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)Giveaways
Increment services
- Antibody Labeling Customized Service
- Protein A/G Purification Column
- Staining Solution for Cells and Tissue
- Positive Control for Antibody
- Tissue/Sections Customized Service
- Phosphorylated Antibody Customized Service
- Western Blot (WB) Experiment Service
- Immunohistochemistry (IHC) Experiment Service
- Immunocytochemistry (ICC) Experiment Service
- Flow Cytometry (FCM) Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Immunofluorescence (IF) Experiment Service
- Buffer
- DAB Chromogen Kit
- SABC Kit
- Long-arm Biotin Labeling Kit
- Real Time PCR Experimental Service
Citations
- Effect of carotid endarterectomy on brain damage markersPubmed:27126899
- Effect of carotid endarterectomy on brain damage markers.pubmed:27126899
- Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implicationsPubmed: 31254929
- Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia33893614
- Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis34678870
- Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients …Pubmed:35566558