Monoclonal Antibody to Neurotrophin 3 (NT3)
NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor
Overview
Properties
- Product No.MAA106Hu24
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsIHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryCytokineNeuro science
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number D3
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA106Hu02-Recombinant Neurotrophin 3 (NT3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Specifity
The antibody is a mouse monoclonal antibody raised against NT3. It has been selected for its ability to recognize NT3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- CaracterizaTeses: Disponiveis
- Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factorpubmed:28388365
- Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso:
- Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered graftsPubmed: 31899965
- Macrophage-derived neurotrophin-3 promotes heterotopic ossification in ratsPubmed: 31896816
- Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡33389158
- Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
- Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
- Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice34193676
- Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377