Monoclonal Antibody to Occludin (OCLN)
Tight Junction Protein Occludin TM4 Minus
Overview
Properties
- Product No.MAC228Ra25
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategorySignal transduction
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG, Clone Number C5
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against OCLN. It has been selected for its ability to recognize OCLN in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.1-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Homo sapiens (Human)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9ScienceDirect: S0006899311021408
- Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and InflammationPubMed: PMC3688973
- A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adultsPubMed: 25794137
- Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
- Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Strokearticles:srep40331
- Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …Pubmed:29375374
- Fas activation alters tight junction proteins in acute lung injuryPubmed: 30385692
- The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned PigletsPubmed: 31426309
- Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumorsPubmed: 32884584
- Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)
- A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and …34492054
- Higher serum occludin after successful reperfusion Is associated with early neurological deteriorationPubmed:35338575
- Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …