Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) Homo sapiens (Human) Monoclonal antibody

POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number 2#
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) Packages (Simulation)
  • Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) Packages (Simulation)
  • Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) AF488 staining on IF;
    Sample: HEL92.1.7 cell
    Primary Ab: 20µg/ml Mouse Anti-Human OCT4 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against OCT4. It has been selected for its ability to recognize OCT4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • A universal glucometer-based biosensor for portable and quantitative detection of transcription factorsRsc: Source
  • Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinomaDoi: 10.3892/ol.2018.9381
  • Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin
  • Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products
  • Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a …Pubmed: 31862609
  • Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effectPubmed:35187753

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