Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) Homo sapiens (Human) Monoclonal antibody

BZS; MHAM; MMAC1; PTEN1; TEP1; Mutated In Multiple Advanced Cancers 1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C3
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPF822Hu01-Recombinant Phosphatase And Tensin Homolog (PTEN)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) Packages (Simulation)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) Packages (Simulation)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) DAB staining on IHC-P; Sample: Human Cerebellum Tissue; Primary Ab: 20µg/ml Mouse Anti-Human PTEN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human PTEN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human PTEN Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) DAB staining on IHC-P;
    Sample: Human Cerebellum Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human PTEN Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAF822Hu23.jpg Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Rabbit Cerebrum lysate Primary Ab: 0.05µg/ml Mouse Anti-Human PTEN Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Hela cell lysate
    Primary Ab: 0.2µg/ml Mouse Anti-Human PTEN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Phosphatase And Tensin Homolog (PTEN) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Porcine Cerebrum lysate
    Primary Ab: 3µg/ml Mouse Anti-Human PTEN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against PTEN. It has been selected for its ability to recognize PTEN in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)

Giveaways

Citations

  • Elucidating the mechanism of miRNA‑214 in the regulation of gingival carcinoma10.3892/etm.2017.4264
  • In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the …Pubmed:35326689
  • Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network

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