Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) Mus musculus (Mouse) Monoclonal antibody

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB972Mu01-Recombinant Pigment Epithelium Derived Factor (PEDF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) Packages (Simulation)
  • Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) Packages (Simulation)
  • Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) DAB staining on IHC-P; Sample: Mouse Stomach Tissue; Primary Ab: 20µg/ml Mouse Anti-Mouse PEDF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Mouse PEDF Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB972Mu22.jpg Western Blot; Sample: Lane1: Rat Kidney lysate; Lane2: HepG2 cell lysate Primary Ab: 2µg/ml Mouse Anti-Mouse PEDF Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) Western Blot; Sample: HepG2 cell lysate
    Primary Ab: 0.1µg/ml Mouse Anti-Mouse PEDF Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of LesionsNature: 2010144
  • Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic NephropathyKarger: 314326
  • Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injuryOxfordJournals: source
  • The change of serum level of pigment epithelium-derived factor in coronary heart diseaseBmj: Source
  • Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK PhosphorylationPubmed: 24288442
  • The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population.Pubmed: 24192856
  • Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent MechanismEndocrine: Source
  • Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsisPubMed: 25700221
  • Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-RPubmed:27187377
  • The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cellsarticleid:2582822
  • VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic micepubmed:28091556
  • 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法201707007
  • PEDF Reduces the Severity of Herpetic Simplex Keratitis in MicePubmed:30025136
  • 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响2018/2/201802020.pdf
  • Lactation-related changes in tissue expression of PEDF in dairy cowsPubmed:29758402
  • Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a …Pubmed:29574467
  • Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosisDoi: 10.1016/j.chemosphere.2018.10.100
  • Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat ModelPubmed: 30392020
  • Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangementPubmed: 30536192
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With HyperlipidemiaPubmed: 31711388
  • Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cellsPubmed: 32315827
  • The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells33498962
  • Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­33768511

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