Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) Rattus norvegicus (Rat) Monoclonal antibody

FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG3 Kappa, Clone Number C5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPF880Ra01-Recombinant Secreted Frizzled Related Protein 1 (SFRP1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) Packages (Simulation)
  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) Packages (Simulation)
  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) DAB staining on IHC-P; Sample: Rat Uterus Tissue; Primary Ab: 30µg/ml Mouse Anti-Rat SFRP1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) DAB staining on IHC-P;
    Sample: Rat Cardiac Muscle Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Rat SFRP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) DAB staining on IHC-P;
    Sample: Rat Testis Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Rat SFRP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) DAB staining on IHC-P;
    Sample: Rat Liver Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Rat SFRP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against SFRP1. It has been selected for its ability to recognize SFRP1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitisPubMed: 23025387
  • Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere FormationPubMed: 26712519
  • Correlation of blood bone turnover biomarkers and Wnt signaling antagonists with AS, DISH,OPLL, and OYL.pubmed:28153008
  • Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial.pubmed:28178355
  • Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial10.1371
  • PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathwaypubmed:28645312
  • Low serum levels of DKK2 predict incident low‐impact fracture in older womenDoi: 10.1002/jbm4.10179
  • Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathwayPubmed: 32041953
  • sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathwayPubmed: 32238888

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