Monoclonal Antibody to Sequestosome 1 (SQSTM1)

OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 111.00 US$ 259.00 US$ 370.00 US$ 925.00 US$ 3,700.00
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Overview

Properties

Product No.MAD198Hu21
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG3 Kappa, Clone Number D19
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPD198Hu01-Recombinant Sequestosome 1 (SQSTM1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Sample: Human Hela cell; Primary Ab: 20µg/ml Mouse Anti-Human SQSTM1 Antibody Second Ab: 5µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human SQSTM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human SQSTM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Mouse Anti-Human SQSTM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: 293T cell lysate Primary Ab: 2μg/ml Mouse Anti-Human SQSTM1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Human Placenta lysate
Primary Ab: 0.05µg/ml Mouse Anti-Human SQSTM1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against SQSTM1. It has been selected for its ability to recognize SQSTM1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

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Citations

Lysosome activation in peripheral blood mononuclear cells and prognostic significance of circulating LC3B in COVID-1933620066
BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY?¡