Monoclonal Antibody to Trefoil Factor 2 (TFF2) Rattus norvegicus (Rat) Monoclonal antibody

SML1; SP; Spasmolytic Protein 1; Spasmolysin; Spasmolytic polypeptide

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number 1-2#
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA748Ra01-Recombinant Trefoil Factor 2 (TFF2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Trefoil Factor 2 (TFF2) Packages (Simulation)
  • Monoclonal Antibody to Trefoil Factor 2 (TFF2) Packages (Simulation)
  • Monoclonal Antibody to Trefoil Factor 2 (TFF2) DAB staining on IHC-P; Sample: Rat Stomach Tissue; Primary Ab: 30µg/ml Mouse Anti-Rat TFF2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19) Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/mL HRP-Linked Capr
  • MAA748Ra21.jpg Figure. Western Blot; Sample: Recombinant TFF2, Rat.
  • Monoclonal Antibody to Trefoil Factor 2 (TFF2) Western Blot; Sample: Rat Pancreas lysate
    Primary Ab: 2µg/ml Mouse Anti-Rat TFF2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against TFF2. It has been selected for its ability to recognize TFF2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in ChinaBiomedcentral: Source
  • Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition.pubmed:29028798
  • Exocrine tissue-driven TFF2 prevents apoptotic cell death of endocrine lineage during pancreas organogenesisPubmed: 30733468
  • ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanismPubmed: 31278375
  • Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
  • Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­

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