Monoclonal Antibody to Ubiquitin (Ub) Homo sapiens (Human), Rattus norvegicus (Rat) Monoclonal antibody

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number D2
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA164Mi02-Recombinant Ubiquitin (Ub)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Ubiquitin (Ub) Packages (Simulation)
  • Monoclonal Antibody to Ubiquitin (Ub) Packages (Simulation)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Samples: Human Tonsil Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Samples: Human Endometrial cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA164Mi22.jpg Western Blot; Sample: Human Lung lysate; Primary Ab: 3µg/ml Mouse Anti-Multi-species Ub Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 3µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) Western Blot; Sample: Rat Liver lysate;
    Primary Ab: 3µg/ml Mouse Anti-Multi-species Ub Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against Ub. It has been selected for its ability to recognize Ub in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970
  • Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice34877816

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