Monoclonal Antibody to Ubiquitin (Ub) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Rhesus monkey (Simian), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus) Monoclonal antibody

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number E8
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA164Mi02-Recombinant Ubiquitin (Ub)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Ubiquitin (Ub) Packages (Simulation)
  • Monoclonal Antibody to Ubiquitin (Ub) Packages (Simulation)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P; Sample: Porcine Colon Tissue Primary Ab: 40µg/ml Mouse Anti-Multi-species Ub Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Sample: Porcine Cerebrum Tissue
    Primary Ab: 40µg/ml Mouse Anti-Multi-species Ub Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Sample: Porcine Small intestine Tissue
    Primary Ab: 40µg/ml Mouse Anti-Multi-species Ub Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Ubiquitin (Ub) DAB staining on IHC-P;
    Sample: Porcine Pancreas Tissue
    Primary Ab: 40µg/ml Mouse Anti-Multi-species Ub Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against Ub. It has been selected for its ability to recognize Ub in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970
  • Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice34877816

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